Gene Reporter Systems
Promega pBIND-GR Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Promega Dual-Luciferase™ Reporter (DLR™) Assay System Passive Lysis 5X Buffer
Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Dual-Luciferase(R) Assay.
Promega NanoLuc™ pNL3 Vectors
Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of binding site or response element not in need of basic promoters
Promega GTPase-Glo Assay, 1,000 assays
The GTPase-Glo Assay assesses the activities of GTPases, GAPs and GEFs, which are components of the GTPase cycle, by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction.
Promega Proteasome-Glo™ Cell-Based Assays
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega CDK2/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK2 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega EnduRen™ Live Cell Substrate
An automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications.
Promega SGK1 Kinase Enzyme System
Recombinant human SGK1 (amino acids 60-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family.
Promega BTK Kinase Enzyme System
Recombinant full-length human BTK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation.
Promega FYN A Kinase Enzyme System
Full-length recombinant human FYN A was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FYN A (Fyn isoform A) is a member of the SRC tyrosine kinase oncogene family showing high homology to YES1, FGR and SRC.
Promega FLT1 Kinase Enzyme System
Recombinant human FLT1 (amino acids 784-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FLT1, also known as fms-like tyrosine kinase 1, is a member of the VEGFR family and is related to the oncogene ROS.
Promega GTPase-Glo Assay, 10,000 assays
The GTPase-Glo Assay assesses the activities of GTPases, GAPs and GEFs, which are components of the GTPase cycle, by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction.
Promega PKCι Kinase Enzyme System
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA), 400 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega ROCK1 Kinase Enzyme System
Recombinant human ROCK1 (amino acids 17-535) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ROCK1 is a ubiquitously expressed serine-threonine kinase that is a downstream target of the small GTPase RhoA.